I presume you mean when flashing to a FlashBoy\Plus? If so, yes.
Try using different usb ports and use the included USB cable and it should work.
My PC has several USB ports, and only one of them works with the cart/software. I also tried using a different USB cable with it in the correct port, and it gave the same error. When using the included USB cable in the correct port, it flashed with no errors.